· Place the hemocytometer under the microscope, in such a way that you see the first small square of the top big square in the middle of your field of view: Start counting your cells. Yes, get your tally counters (or iPhone / Android), feel like those air hostesses checking that everyone’s in, and don’t miss any of your cells! This surface has a grid etched into it. Be sure to clean the hemocytometer and the coverslip before beginning using a little water or alcohol followed by drying with a kimwipe. 2. Prepare an incubation chamber (large petri dish with supports for hemocytometer and wet paper in the bottom). 3. Dilute sperm with water. Place the hemocytometer on the stage of a binocular light microscope. Adjust the microscope to 10X magnification and focus on the cells. Using a hand tally counter, count the cells (stained nuclei) in each of the four outside squares of the hemocytometer (Figure 1A), including cells that lie on the bottom and left-hand perimeters, but not those that lie on the top and right-hand .
We would like to show you a description here but the site won’t allow us. Once you get the cell numbers in the first square, you go to the second (the one on the top right) and do the same thing. Remember, if you counted the cells on the top and right sides but not the ones on the bottom and left sides of the first square, then you must do the same thing with the second one. Place the hemocytometer on the stage of a binocular light microscope. Adjust the microscope to 10X magnification and focus on the cells. Using a hand tally counter, count the cells (stained nuclei) in each of the four outside squares of the hemocytometer (Figure 1A), including cells that lie on the bottom and left-hand perimeters, but not those that lie on the top and right-hand perimeters (Figure 1B).
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